Aim 2

Aim 2 Linking Natural Chemical Variation in Root Exudates to Rhizobiomes

Members

Durham Brooks*, Schachtman*, Adamec, Doyle, Herr, Russo, Shaffer, Twigg, Van Dijk, Weber

*Co-leads

  • Maize exPVP hybrids growing at the Lemnatec Innovation Campus Greenhouses
  • Corn plant in NIC Greenhouse
  • Microscope on lab table

The detailed metabolomic and transcriptomic characterization of the root from Aim 1 will be correlated with root exudate production and microbiome composition in this aim.

Objectives

  1. Root exudate characterization in maize: mass spectrometry and HPLC
  2. Exploring spatial distribution of exudates with colorimetric analysis
  3. Associating changes in the root and rhizobiome with variation in root exudates
  4. Identify the exudate-stimulated rhizobiome and quantify the feed-back on plant phenotype

Associated Publications

Scully, E. D., T. Gries, G. Sarath, N.A. Palmer, L. Baird, M.J. Serapiglia, B.S. Dien, A.A. Boateng, Z. Ge, D.L. Funnell-Harris, P. Twigg, T.E. Clemente, and S.E. Sattler.. 2016. Overexpression of the SbMyb60 impacts phenylpropanoid biosynthesis and alters secondary cell wall composition in Sorghum bicolor. Plant J. 85:378-395
DOI: 10.1111/tpj.13112

Wang, H., C. Zhang, Y. Dou, B. Yu, Y. Liu, T. Heng-Moss, G. Lu, M. Wachholz, J. Bradshaw, P. Twigg, E.Scully, N. Palmer, and G. Sarath.. 2017. Insect and plant-derived miRNAs in greenbug (Schizapis graminum) and yellow sugarcane aphid (Sipha flava) revealed by deep sequencing. Gene. 599: 68-77
DOI: 10.1016/j.gene.2016.11.014