Aim 2

Aim 2 Linking Natural Chemical Variation in Root Exudates to Rhizobiomes

Members

Durham Brooks*, Schachtman*, Adamec, Doyle, Herr, Russo, Shaffer, Twigg, Van Dijk, Weber

*Co-leads

  • Maize exPVP hybrids growing at the Lemnatec Innovation Campus Greenhouses
  • Corn plant in NIC Greenhouse
  • Microscope on lab table

The detailed metabolomic and transcriptomic characterization of the root from Aim 1 will be correlated with root exudate production and microbiome composition in this aim.

Objectives

  1. Root exudate characterization in maize: mass spectrometry and HPLC
  2. Exploring spatial distribution of exudates with colorimetric analysis
  3. Associating changes in the root and rhizobiome with variation in root exudates
  4. Identify the exudate-stimulated rhizobiome and quantify the feed-back on plant phenotype

Associated Publications

Morgan R. McPherson, Peng Wang, Ellen L. Marsh, Robert B. Mitchell, Daniel P. Schachtman.. 2018. Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments. J. Vis. Exp. (137)
DOI: doi:10.3791/57932

Doan, T.H.,* Doan, T.A., Kangas, M.J., Ernest, A.E.,* Tran, D.,* Wilson, C.L., Holmes, A.E., Doyle, E.L., and Durham Brooks, T.L.. 2017. A low-cost imaging method for the temporal and spatial colorimetric detection of free amines on maize root surfaces. Frontiers in Plant Science, 8, 1513.
DOI: https://doi.org/10.3389/fpls.2017.01513

Donze-Reiner, T., N.A. Palmer, E. Scully, T. Prochaska, K, Koch, T. Heng-Moss, J. Bradshaw, P. Twigg, K. Amundsen, S. Sattler, & G. Sarath. . 2018. Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs. BMC Plant Biol. Vol 17, p. 1-21.
DOI: https://doi.org/10.1186/s12870-017-0998-2

Scully, E. D., T. Gries, G. Sarath, N.A. Palmer, L. Baird, M.J. Serapiglia, B.S. Dien, A.A. Boateng, Z. Ge, D.L. Funnell-Harris, P. Twigg, T.E. Clemente, and S.E. Sattler.. 2016. Overexpression of the SbMyb60 impacts phenylpropanoid biosynthesis and alters secondary cell wall composition in Sorghum bicolor. Plant J. 85:378-395
DOI: 10.1111/tpj.13112

Wang, H., C. Zhang, Y. Dou, B. Yu, Y. Liu, T. Heng-Moss, G. Lu, M. Wachholz, J. Bradshaw, P. Twigg, E.Scully, N. Palmer, and G. Sarath.. 2017. Insect and plant-derived miRNAs in greenbug (Schizapis graminum) and yellow sugarcane aphid (Sipha flava) revealed by deep sequencing. Gene. 599: 68-77
DOI: 10.1016/j.gene.2016.11.014